Upgrading to BioWin 6.0

When upgrading from BioWin 5.3 to BioWin 6.0 the link below will provide you with a full installation, including the latest device drivers. Please note that this upgrade will not affect older BioWin versions (BioWin 5.3 and earlier) which are separate applications. This means that users can keep their older copies of BioWin.

BioWin 6.0

NOTE : if you get a flag like the one at right during the download process, this threat detection is a false positive. Microsoft was notified, and have updated Windows Defender. Please ensure that the definition database is version 1.295.1460.0 or greater.  You can update the definition database on this web page: https://docs.microsoft.com/en-us/windows/security/threat-protection/windows-defender-antivirus/windows-defender-security-center-antivirus#definition-version. Follow the directions in the section “Review the definition update version and download the latest updates in the Windows Security app“. This should allow you to proceed with the installation of BioWin 6.0.


  • Annual and Academic License customers can upgrade free of charge at any time via the link above.
  • Perpetual License customers who purchased after June 1, 2018 will be emailed instructions on how to electronically upgrade to BioWin 6.0 free of charge. If you have not received your upgrade email, please contact us. Customers who purchased BioWin prior to June 1, 2018 may order an upgrade here for US$3,300.

BioWin updater will not automatically upgrade BioWin 5.3 to BioWin 6.0. You MUST run the full installation from the link provided. Thereafter the updater will always be checking that your files are up to date.

Previous Versions

Below you will find a list of previous versions of BioWin and PetWin. If you wish to continue running the version you already have and need the latest released version of it, or require an earlier version for a specific project, you can find these here :


  1. Click the download button.
  2. Click Save to save the installation file to your computer.
  3. When the download is complete locate the installation file bw60setup.exe and run it.

Do not plug in your SafeNet Key until the installation is complete. BioWin 6.0 requires a License Activation Code – when you first run the program you’ll be prompted to enter the code. For annual license and academic customers, your current activation code should be valid for BioWin 6.0, simply enter and apply this code to activate BioWin 6.0. For purchase customers, you can click here to order the upgrade to 6.0.

New Developments in BioWin 6.0

New features in BioWin 6.0 can be divided into two main areas : Model Additions and Usability Upgrades. Details are below – or if you prefer, you can download the printable or presentation versions of “What’s New in BioWin 6.0“.


Model Additions

Usability Upgrades


    The BioWin Manual is provided in two forms:

    • As a PDF (default install location is C:\Program Files(x86)\EnviroSim\BioWin 6.0\Manuals)
    • In “Windows Help” format from within BioWin

    To Use PDF

    • Open it from directory above
    • Or copy it to any other location of your choice (g. Desktop, My Documents)

    To Use Windows Help

    • Select Help > Contents & Index
    • Click Help button
    • Press F1 key on your keyboard (context-sensitive method; will open a relevant topic in the manual)

    Example Manual reference:

    For more details on topics, references to relevant sections of the BioWin Help Manual are provided

    MODEL REFERENCE : Chemical Precipitation Reactions > Chemical Phosphorus Removal with Iron Salts

    Model Addition – Sulfur

    Extension of what has been in PetWin for several years

    • Sulfide-oxidizing bacteria
    • Sulfate-reducing bacteria (multiple types)
    • Potential hydrogen sulfide stripping
    • Iron-sulfide (FeS) precipitation
    • Model iron addition for H2S

    MANUAL REFERENCE : Model Reference > Biological/Chemical Models > Sulfur Modeling

    Model Addition – Industrial Organics

    Based on original Alison Baker PhD (~1994, McMaster)

    • Four new state variables for industrial components
    • Mixed removal pathways – stripping and/or biodegradation
    • Default settings for industrial components mimic xylene, phenol, benzene, and toluene
    • Biodegradation according to inhibitory Haldane kinetics

    MANUAL REFERENCE : Model Reference > Modeling of Industrial Components

    Model Addition – Chemical Phosphorus

    New P removal mechanisms (hydrated metal oxides)

    • Based on EnviroSim-sponsored research
    • Extensive calibration for wastewater systems
    • Overcomes weaknesses of old “WEF model” (e.g. fixed Me:P stoichiometry)
    • Can have simultaneous ferric / ferrous / alum inputs

    MANUAL REFERENCE : Model Reference > Chemical Precipitation Reactions > Chemical Phosphorus Removal with Aluminum Salts

    Model Addition – Chemical Phosphorus

    Updated P removal mechanisms (hydrated metal oxides)

    MANUAL REFERENCE : Model Reference > Chemical Precipitation Reactions > Chemical Phosphorus Removal with Iron Salts

    Model Addition – CEPT

    Option to model colloidal COD / metal interactions

    • Model can convert non-settleable colloidal COD to settleable particulate COD in the presence of hydrated metal oxides (HMO)
    • Process will also reduce potential adsorption of P on HMO
    • Ideal or model clarifier can then be used to mimic increased solids and BOD removal

    MANUAL REFERENCE : Model Reference > Chemical Precipitation Reactions > Modeling Metal-Colloidal Coagulation Reactions

    Model Addition – Iron RedOx

    Option to model iron oxidation / reduction reactions

    • New ferrous input (as either ferrous chloride or ferrous sulfate)
    • Ferrous oxidized to ferric in aerobic environments
    • Ferric reduced to ferrous in anaerobic environments
    • Option to track iron-based precipitates {e. FeS and vivianite [Fe3(PO4)2]}

    MANUAL REFERENCE : Model Reference > Chemical Precipitation Reactions > Iron RedOx Reactions and Precipitation of Vivianite and FeS

    Model Addition – Options for P Recovery

    Improved tracking of Ca2+ and Mg2+

    • Previously Ca2+ and Mg2+ only available throughout the flowsheet via input of soluble influent concentrations
    • Underestimated the amounts entering digesters via solids streams limiting the amounts of potential precipitates
    • Model updated to include Ca2+ and Mg2+ in biomass (taken up as part of “synthesis ISS”) and influent degradable solids (XSP)

    Additional sinks for P

    • Vivianite formation
    • Struvite formation as in previous versions of BioWin but no longer Mg2+ limited!
    • Calcium phosphate precipitate (as Brushite)
    • Lowers return stream soluble PO4 to levels typically observed

    MANUAL REFERENCE : Model Reference > Chemical Precipitation Reactions > Precipitation of Brushite, Hydroxy-Apatite and Struvite

    Model Addition – Cellulose

    Cellulose tracking

    • Influent unbiodegradable particulate material split into two components: cellulose and non-cellulose
    • Separate COD:VSS ratios for each
    • Helps to fine-tune sludge production and digester performance
    • Enables modelling of cellulose recovery


    1. Model Reference > Biological/Chemical Models > Activated Sludge Processes > Growth and Decay of Ordinary Heterotrophic Biomass > Stoichiometric Parameters
    2. Model Reference > Definition of Non-State Variables

    Model Addition – SSO

    New input for SSO

    • State variable (CODP-XEO) for adding particulate degradable COD (g. Source Separated Organics)
    • Has specific COD:VSS; added as a separate input to avoid conflicts with municipal wastewater characteristics
    • Option to include N and P
    • Constant or time-varying, as with any BioWin input


    1. Model Reference > Biological/Chemical Models > Hydrolysis, Biological adsorption, Ammonification and Assimilative denitrification
    2. Model Reference > Definition of Non-State Variables

    Usability Upgrade – Drawing Toolbar

    • UNDO button!! Use for accidental element deletion, moving,
    • Buttons for copying pipe format from one pipe to others
    • Buttons for aligning flowsheet elements (vertical or horizontal centers)
    • Buttons for spacing flowsheet elements evenly (vertically or horizontally)
    • Buttons for flipping flowsheet element images (horizontally or vertically)
    • Button for copying selected element(s)

    MANUAL REFERENCE : General Operation > Main Simulator Window > Toolbars > Flowsheet Tools

    Usability Upgrade – Report to Excel

    • Automatic rapid generation and export of data, charts, to Excel
    • Ideal for generating Mass Balance tables to use in PFDs
    • Preconfigured templates are customizable
    • Can incorporate “post-BioWin” calculations (g. MLVSS/MLSS, COD/BOD) using Excel formulas
    • Option for including both steady state and dynamic simulation databases
    • Can include BioWin charts – these are converted to Excel charts with data
    • Report to Word still available

    MANUAL REFERENCE : Data Output (charts, tables, reports) > Creating Project Reports > Creating an Excel Report

    Usability Upgrade – Variable Naming & Sorting

    • Variables and parameters renamed for improved consistency
    • Allows for simpler alphabetical sorting

    MANUAL REFERENCE : Model Reference > Definition of Non-State Variables

    Usability Upgrade – Flowsheet Tags

    Customizable element-specific information

    • Information updates with simulations
    • Can contain state, combined, and element-specific variables

    • Can control location (above, below elements)
    • Can replace flowsheet icons if desired


    1. General Operation > Customizing BioWin > Customizing the Project Appearance > Drawing Board
    2. General Operation > Customizing BioWin > Customizing the Work Environment > Default tags
    3. General Operation > Managing BioWin Projects > Setting Project Options > Drawing Board Options

    Usability Upgrade – Integrated Influent Specifier

    Usability Upgrade – Transpose Tables

    Usability Upgrade – Optional Alarms

    • As part of the project options, select which alarms are active

    MANUAL REFERENCE : General Operation > Managing BioWin Projects > Setting Project Options > Alarm Options

    Usability Upgrade – Set All Parameters to Default

    Use to upgrade older models to Version 6

    Specify which parameter sets to update

    MANUAL REFERENCE : General Operation > Managing BioWin Projects > Specifying Project Model Parameter Values

    Usability Upgrade – Solution Densities

    For metal salt input streams

    MANUAL REFERENCE : Model Reference > Chemical Precipitation Reactions

    Usability Upgrade – Additional Flow Units

    MANUAL REFERENCE : General Operation > Managing BioWin Projects > Setting Project Options > Unit System Cubic meters per hour option for smaller systems

    Usability Upgrade – $ per wet tonne sludge costs

    Third sludge disposal cost option added

    MANUAL REFERENCE : Operating Costs in BioWin > Operating Costs > Sludge

    Usability Upgrade – More Examples

    BioWin Cabinet reorganized and greatly expanded with additional examples